What is the correct evacuated tube order of draw?

Standard order of draw: BLOOD CULTURES, royal blue, red, light blue, SST (Gold), green, tan, yellow, pink, pearl, lavender. If a coag tube (light blue) is the only tube or the first tube to be drawn, a 5 mL discard tube must be drawn first. or incubation conditions. incubation may be required.

Why is there an order of draw for evacuated tubes?

The most current Clinical and Laboratory Standards Institute (CLSI) guidelines recommends a single “Order of Draw”, whether using a multisample, evacuated tube system or drawing with a syringe(s). The “Order of Draw” is designed to eliminate the possibility of cross contamination that may result in erroneous results.

Which tubes needs to be inverted 3 times?

Sodium Citrate Tubes/Coagulation (Light Blue Top) Gently invert the tube 3 to 4 times immediately after collection. NOTE: Light Blue top tubes must be allowed to fill completely; the ratio of blood to anticoagulant is critical.

Which evacuated tube should be drawn first?

first tube to be drawn is a coagulation tube, a discard tube must be drawn first. The discard tube must be a non-additive, no clot activator, or a coagulation tube. NO clot activator or additive tubes should be drawn before coagulation studies.

Does order of draw matter?

In the era of lyophilized anticoagulants, order of draw is no longer important. Contamination of serum samples with K EDTA will occur routinely if order of draw is not followed. During syringe collections, contamination with K EDTA may rarely occur if order of draw is not followed.

How many times should a green tube be inverted?

Light green-top tube (lithium heparin) This tube contains lithium heparin and gel separator used for the collection of heparinized plasma for routine chemistry tests. NOTE: After the tube has been filled with blood, immediately invert the tube 8-10 times to mix and ensure adequate anticoagulation of the specimen.

What is the minimum number of evacuated tubes required to collect a CBC and a PT when using a winged infusion set?

Three tubes of blood need to be collected. The blue and green tubes fill easily and completely, but the lavender tube fills incompletely. Changing the lavender tube using the same draw also results in an incomplete specimen.

What does SST stand for in phlebotomy?

Serum separator tube (SST) contains a. gel at the bottom to separate blood cells. from serum on centrifugation.

Which vein is preferred by most phlebotomists?

Major arm veins used for phlebotomy. The median cubital vein is the larger and more stable vein and is preferred for venipuncture.

What can happen if you use the wrong order of draw?

Contamination of serum samples with K EDTA will occur routinely if order of draw is not followed. During syringe collections, contamination with K EDTA may rarely occur if order of draw is not followed.

Do SST tubes need to be centrifuged?

To obtain serum, whole blood should be collected into either an SST or plain red top tube. After collection, allow the tube to sit in an upright position at room temperature for 30-45 minutes. After the clot has formed, centrifuge the tube for 10 minutes at 3400 rpm.

What color tube is SPS?

14. Yellow Top Tube Additive: Contains the anticoagulant sodium polyanetholesulfonate (SPS). Used to collect specimens to be cultured.

What are the 3 most common veins used in phlebotomy where are they located?

There are three veins most commonly used in venipuncture, or phlebotomy. They are the cephalic, median cubital, and basilic veins. These three veins are found in the antecubital area. The cephalic vein is found on the lateral, or outside, of the arm.