What is contact plate method?
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What is contact plate method?
Method for making the bacterial load on surfaces visible. A nutrient Agar surface is pressed against the test surface for a short moment. Thus, part of the bacteria colonising the surface is transferred to the agar surface.
What is the purpose of a contact plate?
Contact plates are the standard devices for environmental monitoring of surfaces and personnel in cleanrooms and isolators.
What is spread plate?
The spread plate method is a technique to plate a liquid sample containing bacteria so that the bacteria are easy to count and isolate. A successful spread plate will have a countable number of isolated bacterial colonies evenly distributed on the plate.
What is the difference between TSA and SDA plates?
SDA is inappropriate as a general purpose environmental medium and should only be used to quantify moulds and yeasts. TSA incubated at 25°C will recover moulds and yeasts and the inclusion of 1% glucose does appear to have benefits in improving their recovery to the same level as SDA.
How long do agar plates take to set?
*Pro-Tip* It takes roughly 30 min for our plates to solidify at room temperature, however we leave them out at room temperature overnight to allow them to dry. After overnight drying, we place the plates in a plastic bag with an absorbent material to reduce condensation. The plates are then stored at 4 ℃ until use.
How much do agar plates cost?
| Catalog No. | Plate Type | Price/Plate |
|---|---|---|
| 4001-100 | LB Agar Plates, 100x15mm | $1.50 |
| 4001-150 | LB Agar Plates, 150x15mm | $2.50 |
| 4002-100 | LB Agar w/ Ampcillin 100ug/ml Plates*, 100x15mm | $1.50 |
| 4002-150 | LB Agar w/ Ampcillin 100ug/ml Plates*, 150x15mm | $2.50 |
How do you make an agar plate in aseptic laboratory?
To ensure the loop is not too hot, touch it lightly on the agar plate in a designated area where no streaking will occur. Only a few cells are needed from the colony, not the entire colony. If using a metal loop, flame it using a Bunsen burner before obtaining the inoculum for the plate (Panel B of Figure 3 ).
When to use spread or pour plate for microbial testing?
Use a spread or pour plate (psychrotrophs may not survive as well in pour plates) that includes homogenized food sample. Incubation is aerobic at 35oC for 48± 2 h. APC–aerobic plate count – cells spread over agar surface and incubated aerobically.
How to count the number of microbes in a food sample?
A few can allow you to count the number of microbes from a specific genus like Salmonella. 1. Standard Plate Count (SPC)By far the most widely used method for determining the number of viable colony forming units (CFU) in a food. Use a spread or pour plate (psychrotrophs may not survive as well in pour plates) that includes homogenized food sample.
What are the methods for plating bacterial colonies?
Procedures described include (1) streak-plating bacterial cultures to isolate single colonies, (2) pour-plating and (3) spread-plating to enumerate viable bacterial colonies, (4) soft agar overlays to isolate phage and enumerate plaques, and (5) replica-plating to transfer cells from one plate to another in an identical spatial pattern.